Purified Anti-Mouse CD16/32 Antibody[93], Functional Grade (AN009280)
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For research use only.
| Alternate Names | Fcgr, Lymphocyte antigen, Fc gamma receptor IIB, Fc-gamma RII, FcRII, Lymphocyte antigen 17, CD32, Fcgr2, Fcgr2b, Ly-17, CD16, Fc-gamma-RIIB, FcRII), Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB (Fc-gamma RII, IgG Fc receptor II beta, Lymphocyte antigen 17 (Ly-17), Fc&, gamma-RIII-I, Fc&, gamma-RII-I, Ly-17, FcgRIIIA |
| Clone | |
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution | FCM ≤ 0.2 µg per million cells in 100 µL volume;Block Reported in the literature |
| Background | Fc gamma RII, also named CD32, is a low-affinity receptor for the Fc region of complexed IgG. Three genes for human Fc gamma RII (A, B and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins, have been identified. The extracellular domain of mouse Fc gamma RII shares approximately 95% amino acid sequence identity with that of mouse Fc gamma RIII/CD16. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Mouse CD16/32 protein |
| Abbre | CD16/32 |
| Swissprot | |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Rat IgG2a, κ |
| Isotype Control | Purified Rat IgG2a, κ Isotype Control[2A3], Functional Grade |
| Applications |
FCM;Block
|
| Cellular Localization |
Cell membrane
|
| Concentration |
≥ 3 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Terrazas LI, et al. Int. J. Parasitol. 2005;35:1349. |
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